Fig. 2
Demonstration of cell sorting in the microfluidic T-maze using marine bacteria. a The microfluidic T-maze sorts Marinobacter adhaerens wild type (red) from a nonchemotactic mutant (ΔcheA; blue) in an initial 1:1 mixture as cells migrate through the device. Chemoattractant concentration (liquid 2216 medium) increases from top to bottom in the figure (yellow–red color scale). Diagrams of the junctions show measured cell trajectories. The scale bar shown in the figure is 200 μm. b The sorting index, Sn, the ratio of WT to mutant cells found at each junction. c The choice factor, CF, measuring the tendency of cells to swim up the gradient at each junction (red and blue for wild type and mutant, respectively). Curves in (b) and (c) show mean ± s.d. over three replicates
