Fig. 3 | Nature Communications

Fig. 3

From: Hepatocyte-specific loss of GPS2 in mice reduces non-alcoholic steatohepatitis via activation of PPARα

Fig. 3

GPS2 depletion causes PPARα activation. a Heatmap representing the top 1000 significant (sorted based on adj P value) gene expression in CD-fed WT and LKO livers. b KEGG pathway analysis of significantly up-regulated and down-regulated genes in LKO mice. c, d QPCR analysis of mRNA expression in WT and LKO mice upon (c) fasting, n = 5 in each group; and (d) PPARα agonist (GW7647) treatment, n = 5 in the vehicle groups, n = 7 in the GW7647-treated groups, one-way ANOVA followed by Tukey’s test. e Motif analysis of GPS2-occupied regions in mouse liver. f Co-immunoprecipitation of GPS2 domains with PPARα in 293 cells (* represents non-specific bands). g QPCR analysis of GPS2-regulated genes and (h) fed and fasted serum ketone bodies in PPARα KO (PKO) and liver Gps2/Pparα double KO (PGKO) mice livers, n = 3 in PKO and n = 4 in PGKO, non-parametric Mann–Whitney test. All data are represented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001

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