Fig. 6 | Nature Communications

Fig. 6

From: Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Fig. 6

Illustration of DNA repair outcomes after Cas9-induced double-strand break. Both blunt and staggered ends can be directly ligated back into wild-type sequence, or generate a deletion through resection by nuclease activity prior to ligation. Blunt ends can also result in an insertion by template-independent addition of a random nucleotide, possibly established by Pol µ. Staggered ends lead primarily to template-dependent insertions, possibly established by polymerases such as Pol µ or Pol λ

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