Fig. 7 | Nature Communications

Fig. 7

From: Mitochondria-specific drug release and reactive oxygen species burst induced by polyprodrug nanoreactors can enhance chemotherapy

Fig. 7

In vitro cytotoxicity and the analysis of apoptosis/necrosis. a In vitro cytotoxicity determined by MTT assay against 4T1 cells upon 36 h treatment with five different samples. The mean value was calculated by the t test (mean ± s.e.m. n = 6). *p < 0.05, **p < 0.01, compared with the NT-PNs group. b Cell apoptosis and necrosis analyzed by flow cytometer with Annexin V-FITC/PI double staining after different treatments. c Statistical analysis for the percentage of apoptotic cells and necrotic cells in b. The mean value was calculated by the t test (mean ± s.e.m. n = 3). *p < 0.05, **p < 0.01, compared with the control group. d Confocal images of 4T1 cells upon different treatments and stained with Calcein-AM/PI. Live cells were stained green with calcein AM, and dead/later apoptotic cells were stained red with PI (scale bar, 50 μm)

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