Fig. 1

Sdc2 EC deletion leads to impaired angiogenesis. a–d Retinas from P6 pups were stained with isolectin B4 for specific detection of endothelium (in green). a Representative pictures of retinal vascular outgrowth for each genotype (500 µm scale bars). b Quantification of vascular progression expressed as ratio between length of vascular front and retina edge (n = 8–12 retinas from 4 to 6 mice, each dot corresponds to a different retina). c Representative pictures of vascular branching (100 µm scale bars) and quantification (d) (n = 4–10 retinas from 4 to 5 animals, each dot corresponds to a different retina). e, f PBS or indicated growth factor pellets were inserted in a cornea micro-pocket and angiogenic response was evaluated by CD31 staining (in red) after 1 week. e Representative pictures of cornea-angiogenic responses (100 µm scale bars) and quantification (f) (n = 3 mice for each treatment and genotype, each dot corresponds to a different cornea). Errors bars represent standard error of the mean (SEM). Statistical analysis was performed by one-way Anova with Bonferroni’s multiple comparison test (N.S. not significant, ^**P < 0.01, ^***P < 0.001)