Fig. 2

Influenza particles are found in platelets by confocal microscopy. 50 μL of intravenous blood drawn from a influenza positive patients or healthy donors. Blood was fixed (RBCs were lysed) and samples were later stained with antibodies for the nuclear protein for influenza A (green) or influenza B (green, yellow = green and red combined), for platelets stained with CD41-APC (red), and DNA from neutrophils (white). Arrows point toward the influenza (green) staining. Representative images are shown from n = 10 different patients. Confocal microscopy of b platelets isolated from healthy donors and incubated with WSN/33, at 1 pfu to 100 platelets, for 30 min, at 1000 rpm, 37 °C. Platelets were fixed, permeabilized, and stained with the same antibodies as in (a). Representative images of n = 4 (2F, 2M) are shown