Fig. 2
From: Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
The cryo-EM map of full-length MloK1 at 4 Å. The map was obtained from the consensus single-particle refinement of 2D crystal data in FREALIGN. a The full refined map containing approximately nine MloK1 tetramers; total molecular weight over 1 MDa. b Projection of the full map along the z axis. The sub-volume used for postprocessing is indicated (red box). Insets: projections of the central sub-volume orthogonal to the xy and xz planes. c The postprocessed map of the central tetramer with the refined atomic model fit into the density, viewed from the extracellular side and parallel to the membrane plane. The pale blue dashed lines indicate the approximate position of the lipid bilayer. Scale bars: 50 Å
