Fig. 3 | Nature Communications

Fig. 3

From: Physical and functional interaction between A20 and ATG16L1-WD40 domain in the control of intestinal homeostasis

Fig. 3

ATG16L1 recognizes the N-terminal region of A20 (92–263) through the WDD. a A stretch of A20 including amino acids 1–263 suffices to co-precipitate with GST-ATG16L1. b A minimal region between amino acids 92–263 of A20 suffices to interact with the WDD of ATG16L1. Co-immunoprecipitation assays were done as in Fig. 2. c Endogenous A20 coprecipitates with ATG16L1 upon TNF treatment of Atg16L1−/− MEFs and HCT116 cells restored with HA-ATG16L1. The indicated cells were treated with 30 ng/ml of TNF for 2 h, and processed for anti-ATG16L1 immunoprecipitation and western-blotting with the shown antibodies

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