Fig. 5
IEC-specific A20/Atg16l1 deficient mice develop spontaneous intestinal pathology. a Representative Western blot analysis of small intestinal organoid cultures from wild-type (WT), A20∆IEC, Atg16l1∆IEC, and A20/Atg16l1 dKO mice. b Absolute body weight in grams of WT (n = 18), A20∆IEC (n = 10), Atg16l1∆IEC (n = 7), and littermate A20/Atg16l1 dKO (n = 8) mice in function of time. Data are expressed as mean ± SEM. *p < 0.05 for dKO mice compared to the three control groups, as analysed as repeated measurements using the residual maximum likelihood as implemented in Genstat v17. c Representative picture of 20-week-old littermate WT, A20∆IEC, Atg16l1∆IEC, and A20/Atg16l1 dKO mice. d Body weight of WT, A20∆IEC, Atg16l1∆IEC, and A20/Atg16l1 dKO mice at the age of 5 weeks. Each symbol represents one mouse. Data represent mean±SEM. ****p < 0.0001; ***p < 0.001, as analyzed in GraphPad Prism 7 with Kruskal-Wallis test. e Representative macroscopic colon images of 20-week-old WT, A20∆IEC, Atg16l1∆IEC, and dKO littermate mice showing rectal prolapses and colon thickening in dKO animals. f Representative macroscopic image of the small intestine of a 20-week-old dKO mouse showing increased vascularization and swelling in the jejunum (red arrow head). g Representative endoscopic pictures of 10-week old WT, A20∆IEC, Atg16l1∆IEC, and dKO mice demonstrating spontaneous colon pathology in dKO mice. h, i Colon inflammation detected by endoscopy and represented as mouse endoscopic index of colitis severity (MEICS) score in 10-week-old (h) and 20-week-old (i) WT, A20∆IEC, Atg16l1∆IEC, and dKO mice. Each symbol represents one mouse. Data represent mean±SEM. ****p < 0.0001; ***p < 0.001; **p < 0.01, as analyzed in GraphPad Prism 7 with Kruskal-Wallis test. j Quantitative PCR for TNF, IL-1β, CXCL2, and MCP1 in small intestinal lysates from WT, A20∆IEC, Atg16l1∆IEC, and dKO mice. Each symbol represents one mouse. Data represent mean±SEM. ***p < 0.001; **p < 0.01; *p < 0.05, as analysed in GraphPad Prism 7 with One-way ANOVA
