Fig. 7
From: A DNMT3A PWWP mutation leads to methylation of bivalent chromatin and growth retardation in mice
Genomic landscape of de-repressed genes in Dnmt3a Δ/D329A hypothalamus. a, b Representative genome browser views of gene expression, DNA methylation, and distribution of H3K4me3, H3K27me3, and H3K36me3 marks (determined by ChIP-seq) in adult (14-week) male hypothalamus of Dnmt3a+/+ and Dnmt3aΔ/D329A genotypes. H3K4me3 and H3K36me3 are enriched over actively transcribed CGI promoters and gene bodies, respectively. H3K27me3 shows a broad enrichment over transcriptionally silent genes and loss of enrichment upon methylation gain. Genes a Pou4f1 and b Tal1 are up-regulated and hypermethylated in Dnmt3aΔ/D329A. CGI: CpG island, DMR: hypermethylated region. Each colour-coded block represents: a 50 bp window with 10 bp step size in the gene expression dataset; a 100-CpG window in the methylation dataset; and 2 kb window with 1 kb step size in the histone enrichment datasets. For gene and mRNA tracks, the colour indicates direction, where red is a forward strand and blue is a reverse strand. Error bars indicate standard deviation. n(+/+) = 5, n(Δ/D392A) = 4 for RNA-seq datasets; n = 3 for each genotype in WGBS-seq and ChIP-seq datasets
