Fig. 4
From: KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Depleting KIBRA promotes the degradation of Rab27a. a Heat map of proteins involved in exosome biogenesis or secretion by mass spectrometry in the cortex and hippocampus of three KIBRA-KO mice and two wild-type (WT) controls. Proteins are arranged according to fold change values. Upregulated and downregulated proteins are indicated by red and blue hues, respectively. Color intensity indicates the expression levels of proteins as displayed. b Rab27a was detected by immunofluorescent staining in the cortex and hippocampus of KIBRA-KO mice and WT controls (n = 3 in each group). Scale bar = 200 μm. c Western blot was performed to determine the Rab27a protein levels in the cortex, hippocampus, kidney, muscle, and liver of KIBRA-KO mice and WT controls (n = 3 in each group). d Quantification of Rab27a levels in different tissues of KIBRA-KO and WT mice in three independent experiments. e Real-time PCR was performed to determine the mRNA levels in different tissues of KIBRA-KO and WT mice (n = 3 in each group). The quantification results were plotted as dot plots, showing the mean ± SE. *P < 0.05, **P < 0.01. n.s., not significant (P > 0.05) as determined by two-tailed t test
