Fig. 3 | Nature Communications

Fig. 3

From: PDGFRα+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland

Fig. 3

Endogenous Pdgfrα promoter activity in the mammary gland. a A mouse reporter for endogenous Pdgfrα promoter activity. b Co-staining for H2B-eGFP and PDGFRα in the adult gland (representative of n = 3 mice and 5 fields per tissue section); scale bar = 25 µm. c Flow cytometry histograms depicting H2B-eGFP+ cells (green) in mammary subsets relative to wild-type control (black). d Mean fluorescence intensity (MFI) of H2B-eGFP in (c) (n = 3 mice). e Droplet digital PCR analysis of Pdgfrα, K14, K18 normalized to Gapdh in FACS-sorted GFP+ and GFP- cell fractions from reporter mice (isolated from n = 3 individual mice). Data represent mean ± s.e.m. *p< 0.05, ***p<0.001 (one-way ANOVA). Source data are provided as a Source Data file

Back to article page