Fig. 2
From: Co-translational assembly of mammalian nuclear multisubunit complexes
Sequential assembly of TAF10 and TAF8. N-terminal (a) and C-terminal (c) anti-HA RIP-qPCR of HeLa cell polysome extracts, co-transfected with the corresponding TAF8 and TAF10 expression plasmids (as indicated). N-terminal (b) and C-terminal (d) anti-FLAG RIP-qPCR of HeLa cell polysome extracts, co-transfected with the indicated expression plasmids. PPIB (a–d) was used as negative control mRNA. In panels (a–d) mRNA fold enrichment is expressed as fold change with respect to the mock IP calculated by the formula ΔΔCp [IP/mock] and error bars represent ±SD from two biological and two technical replicates (represented by grey dots). Source data provided as a Source Data File
