Fig. 3
From: Co-translational assembly of mammalian nuclear multisubunit complexes
Protein-protein ID drives co-translational assembly of TAF10 and TAF8. a anti-HA-TAF10 RIP-qPCR of HeLa cells co-transfected with HA-TAF10 and either wild type FLAG-TAF8 (TAF8 wt) or mutant FLAG-TAF8 (TAF8 S57F L65F). b anti-HA-TAF10 RIP-qPCR of HeLa cells co-transfected with HA-TAF10 and either a minimal TAF8 HFD or TAF8 HFD extended with 60 amino acids (TAF8 HFD + 60aa). In panels (a, b) relative mRNA fold enrichment is expressed as fold change of TAF8 mRNA (with respect to the mock IP calculated by the formula ΔΔCp [IP/mock]) relative to TAF10 mRNA and error bars represent ±SD from two biological and two technical replicates (represented by grey dots). Source data provided as a Source Data File
