Fig. 8
From: Co-translational assembly of mammalian nuclear multisubunit complexes
TREX-2 and SAGA DUB complexes also assemble co-translationally. a ENY2 is shared between TREX-2 and SAGA complex. Two protein molecules of ENY2 binds to the large GANP subunit of TREX-2 complex and ENY2 is also a part of the deubiquitination module of the SAGA complex. b RIP-qPCR of GFP IP from HeLa cell polysome extracts stably expressing GFP-ENY2 tagged at the N-terminus. Error bars represent ±SD from two biological replicates and two technical replicates (represented by blue dots). c Western blot analysis of GFP IP from polysome extract prepared from GFP-ENY2 cell line. Molecular weight (MW) markers are shown in kDa. FT flow-through. IP immunoprecipitation. GAPDH was used as unrelated control mRNA. Source data provided as a Source Data File
