Fig. 4
From: Low yield and abiotic origin of N2O formed by the complete nitrifier Nitrospira inopinata
Instantaneous O2 consumption and NO production during NO2− oxidation by N. inopinata. The data shown here represents a single replicate of three biological replicates (n = 3). Two additional biological replicates are shown in Supplementary Fig. 8. Dissolved O2 is shown in open circles, dissolved NO in filled gray triangles, and NO2− in open diamonds. The NO2− concentration immediately after injection (~6 min) were inferred from the injected volume of a stock NaNO2 solution, otherwise NO2− concentrations were determined in three technical replicates (n = 3). Experiments were performed in a 10-mL microrespiration (MR) chamber fitted with an O2 and NO microsensors. The arrow marks the addition of 2.5 mM NO2− into the MR chamber. No NO formation from NO2− was measurable in oxic sterile media controls containing heat-killed biomass. Source data are provided as a Source Data file
