Fig. 3
From: A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
Near-atomic resolution map of the DARPin adaptor and its bound cargo protein GFP. a A density map obtained following multi-body refinement in RELION is shown colored by local resolution in a side view (left) and a sliced view (right), showing the central helix in the center of the GFP beta barrel. b Gold-standard FSC curve for the reconstruction. c Two wall-eyed stereo views of the sharpened GFP density map. The full GFP density is shown at the top; a sliced view with a fitted GFP atomic model is shown at the bottom. The fluorophore and Leu 64 (shown in sticks) in the central helical region have clear surrounding density. d Comparison between the DARPin plus GFP model in this study (GFP in green, DARPin in magenta) and the previously reported 3G124 eGFP crystal structure (grey, PDB 5MA8). The two structures are aligned on the DARPin portion. e Wall-eyed stereo views of the extensive interface between the DARPin (magenta) and GFP (green). The side chains of the bulky residues forming the interface are shown in sticks
