Fig. 4
From: L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction
Structural and functional interactions of Tyr 236. 2Fo-Fc density maps contoured at 1σ for the BasC binding site residues in apo at 2.9 Å (gray) (a) and 2-AIB bound at 3.4 Å (purple) (b) structures. c 10 µM [3H]l-alanine/4 mM l-alanine exchange by wild-type (WT) and Y236F mutant BasC reconstituted in proteoliposomes (black bars) and transport of 10 µM [3H]l-alanine in HeLa cells by human Asc-1 (hAsc-1) WT and Y280F mutant (gray bars). Data (mean ± s.e.m.) normalized to WT values from 3 independent experiments. Source data are provided as a Source Data file
