Table 1 Affinity and selectivity of selected DARPins for ERK2 and pERK2

From: A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

DARPin

KD (M), pERK2

KD (M), ERK2

Selectivity

pE59

453 × 10−9

>3.5 × 10−6

>7.7

pEM1

87.1 × 10−9

>3.5 × 10−6

>40

EpEM6

633 × 10−9

784 × 10−9

1.0

EpEM7

25.2 × 10−9

222 × 10−9

8.8

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