Fig. 4
From: Spatiotemporal control of coacervate formation within liposomes
Sequestration of biomolecules and compartmentalization of reactions within coacervates. The pLL/ATP coacervate (green) formed within the liposome (red) serves multiple purposes, from concentrating proteins and supramolecular assemblies to facilitating enzymatic reactions. a Fluorescence time-lapse images showing coacervation dynamics (upper panel) and simultaneous sequestration of FtsZ (cyan) within the coacervates (lower panel). b SUVs (in white) get sequestered inside the condensates. c The β-galactosidase reaction is getting carried out predominantly within the coacervate. No fluorescent product is observed before the addition of the FDG substrate (t0)—two left panels. Addition of FDG induces the enzymatic reaction inside the liposome, particularly in the condensed phase, as can be seen from the colocalization of the fluorescent product, fluorescein (in magenta) with the coacervate—two right panels. d Fluorescein intensity within the coacervate phase increases about 14-fold in 2 h after the addition of the substrate (n = 31 in both the cases). The circles represent the individual data points, bars represent the mean fluorescence intensity, and the error bars indicate corresponding standard deviations. Source data are provided as a Source Data file
