Fig. 5

Phf19 restricts CD8⁺ T cell terminal differentiation. a Quantitative RT-PCR of Mir155, Ezh2, and Phf19 after transfer of 10⁵ pmel-1 CD8⁺Ly5.1⁺ T cells into wild-type mice in conjunction with gp100-VV assessed at the indicated points. Ezh2 and Phf19 levels are relative to Rpl13, Mir155 levels are relative to U6. b Flow cytometry of splenic live CD8⁺Ly5.1⁺ T cells 5 days after transfer of 3 × 10⁵ naïve pmel-1 CD8⁺Ly5.1⁺ Phf19^+/+ or Phf19^−/− T cells into wild-type mice in conjunction with gp100-VV. Numbers indicate percentage after gating on live CD8⁺Ly5.1⁺ T cells. c Percentage (left) and number (right) of splenic pmel-1 CD8⁺Ly5.1⁺ T_E cells 5 days after transfer as in b. d Percentage (left) and number (right) of splenic CD8⁺Ly5.1⁺CD62L⁺ T cells 5 days after adoptive transfer as in b. Data are presented as box plots extending to minimum and maximum values. Bands inside the boxes represent median values of three individual mice. e ChIP-qPCR using H3K27me3 antibody on in vitro activated non T_E KLRG1⁻ Phf19^+/+ or Phf19^−/− T cells with primers specific to the transcription start site of selected TFs. ChIP enrichments are presented as the percentage of protein bound, normalized to input. Bars represent the mean ± s.e.m. of technical triplicates. Data are representative of two independent experiments. *P < 0.05; **P < 0.01; ****P < 0.001 (unpaired two-tailed Student’s t-test)