Fig. 4
SIRT3 is required for GABAergic synaptic scaling. a Immunoblots (left) showing relative levels of SIRT3 protein in cultured WT hippocampal neurons exposed to the indicated treatments for 24 h; 100 μM picrotoxin (PTX) for 12 h; 100 μM PTX for 24 h; 10 μM EUK-134 plus 100 μM PTX for 24 h. (*P < 0.05). b Whole-cell recordings of mIPSCs in cultured hippocampal neurons from Sirt3+/+ and Sirt3–/– mice that had been treated with vehicle or 100 μM PTX for 24 h. c Amplitudes of mIPSCs (left) and a plot of cumulative event probability as a function of mIPSC amplitude (right) in Sirt3+/+ and Sirt3–/– neurons treated for 24 h with vehicle or PTX (15 neurons/group). d mIPSC frequency (left) and a plot of cumulative event probability as a function of mIPSC inter-event interval (right) in Sirt3+/+ and Sirt3–/– neurons treated for 24 h with vehicle or PTX (15 neurons/group). *P < 0.05 compared to the values for each of the other 3 groups. e Whole-cell recordings of miniature postsynaptic currents (mIPSCs) in cultured hippocampal neurons from Sirt3–/– mice that had been infected with AAV vectors to overexpress either SIRT3 and GFP, or GFP alone; 6 days after infection, cultures were treated with vehicle or 100 μM PTX for 24 h. f Amplitudes (left) and frequencies (right) of mIPSCs in Sirt3–/– neurons in the four different groups (15 neurons/group). *P < 0.05 compared to the values for each of the other 3 groups. g Whole-cell recordings of miniature postsynaptic currents (mIPSCs) in cultured hippocampal neurons from Sirt3+/+ mice that had been treated for 24 h with vehicle, 100 μM PTX, 0.3 μM paraquat (PQ) plus PTX, 1.0 μM PQ plus PTX, or 0.3 μM PQ plus vehicle. h mIPSC amplitudes (left) and frequencies (right) in Sirt3+/+ neurons in the indicated treatment groups (24 h treatment; 12 neurons/group). *P < 0.05. i Results of measurements of mIPSC amplitudes in Sirt3-/- neurons in the indicated treatment groups (24 h treatment; 12 neurons/group). *P < 0.05. All error bars are the SEM. All statistical comparisons for data in this figure were performed using ANOVA and Newman–Keuls post hoc tests for pairwise comparisons. Source data for all graphs in this figure are provided in supplemental Source Data file
