Table 1 Frequency of MP subsets in BAL and blood
From: Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace
MP subset | AM | CD14++ CD16− | CD14++ CD16+ | CD14+ CD16++ | DC1 | DC2/3 | CD1c− | DC5 | pDC | |
|---|---|---|---|---|---|---|---|---|---|---|
Antigen expression by flow cytometry | CD45 | + | + | + | + | + | + | + | + | + |
SSC | hi | lo | lo | lo | lo | lo | lo | lo | lo | |
Lineage | − | − | − | − | − | − | − | − | − | |
HLA-DR | + | + | + | + | + | + | + | + | + | |
CD14 | + | ++ | ++ | + | − | − | − | − | − | |
CD16 | + | − | + | ++ | − | − | − | − | − | |
CD11c | + | + | + | -/+ | − | |||||
CD123 | − | − | − | −/+ | + | |||||
BTLA | ++ | −/+ | − | + | + | |||||
CD1c | +/− | + | − | -/+ | − | |||||
Axl | +/− | +/− | +/− | + | − | |||||
Siglec 6 | − | − | − | + | − | |||||
% of leukocytes mean (SD) % of MPs mean (SD) | HC whole blood | 0 | 8.01 (2.04) | 0.47 (0.22) | 1.29 (0.85) | 0.02 (0.01) | 0.30 (0.1) | 0.07 (0.07) | 0.02 (0.02) | 0.23 (0.05) |
75.0 (7.94) | 6.41 (2.29) | 13.8 (6.11) | 0.23 (0.22) | 1.07 (0.75) | 0.77 (0.72) | 0.22 (0.20) | 1.93 (0.93) | |||
SS BAL | 60.1 (14.3) | 1.06 (0.14) | 2.38 (0.95) | 0.64 (0.56) | 0.12 (0.10) | 0.82 (0.35) | 0.69 (0.72) | 0.04 (0.02) | 0.13 (0.07) | |
2.92 (1.88) | 43.7 (8.00) | 11.4 (7.31) | 2.41 (1.91) | 16.1 (4.24) | 12.3 (13.9) | 1.06 (0.36) | 2.57 (1.53) | |||
LPS BAL | 23.1 (13.5) | 18.7 (9.13) | 4.51 (3.23) | 0.23 (0.16) | 0.09 (0.04) | 1.63 (0.61) | 0.2 (0.11) | 0.14 (0.05) | 0.22 (0.16) | |
64.3 (17.8) | 18.2 (16.6) | 0.91 (0.67) | 0.33 (0.15) | 6.12 (2.59) | 0.69 (0.37) | 0.45 (0.11) | 0.76 (0.49) |
