Fig. 4 | Nature Communications

Fig. 4

From: The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization

Fig. 4

The B. subtilis extracellular matrix functions as a physical barrier. a, b Magnetic resonance imaging (MRI) analysis of Δmatrix and 3610 colonies after 72 h of growth on LB media. ce Characterization by solid-state NMR of 3610 and Δmatrix grown on LB media after 72 h. a Transversal relaxation times (T2) of Δmatrix and 3610 colonies. T2-weighted (gray scale images) and T2 map (heatmap) of Δmatrix and 3610 colonies. b Apparent diffusion coefficient (ADC) of Δmatrix and 3610 colonies. c 13C cross-polarization spectra of 13C-labeled 3610 and Δmatrix samples. d 13C-detected spectra of the 3610 and the Δmatrix samples after a T2-filter and a 1H spin diffusion of 5 ms. e Superimposition of 2D 1H-13C HETCOR experiments of Δmatrix recorded without (in red) and with (in blue) a 1H spin diffusion mixing time. The bulk water frequency is indicated in yellow

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