Fig. 2
Forebrain-specific Cic deletion increases glial cells at the expense of neurons. a Targeting strategy for Cic conditional knockout mice. Exon numbering is shown relative to Cic transcript variant 1. b Forebrain-deletion of Cic starting from E10.5 by crossing CIC-floxed line with FoxG1-cre. CicFl/Fl;FoxG1Cre/+ animals are compared with CicFl/+;FoxGCre/+ or CicFl/Fl;FoxG1+/+ as controls. c Representative gross morphology of Cic-deleted and Cic-wildtype brains at P21. d, e Representative staining d and total quantitation e of NeuN + , Gfap + , and Sox10 + cells in Cic-deleted (CicFl/Fl;FoxG1Cre/+) cortex vs control (CicFl/Fl;FoxG1+/+). Scale bar: 50 μm. f, g Representative staining f and total quantitation g of Olig2 + , Pdgfra + , and CNPase + cells in lateral corpus callosum, and Mbp expression in lateral corpus callosum at P2. Data from n = 5 mice per each group with the exception of Pdgfra staining (n = 3 control, n = 4 Cic-null) and Mbp staining (n = 6). Statistical analyses performed by unpaired t test. Scale bar: 50 μm. Source data are provided as a Source Data file. Data shown as mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001. Labels: Ctx–cortex, CC–corpus callosum
