Fig. 4

Deletion of Cic in the neurogenic period biases NSCs to glial lineage selection. a Localized deletion of Cic in neural stem cells via in utero electroporation into VZ cell of Cic-floxed embryos; targeted cells carry a fluorescent marker and express either cre recombinase (Cre), dominant negative ETV5 (DNETV5), or not (Empty control), depending on the electroporated plasmid. b–l Immunofluorescence staining and quantitation of cells in the electroporated patch. Sox2 + stem cells b, c and Sox9 + glioblasts d, e in n = 4 per group 2 days after E13 electroporation, analyzed at E15. Late-born Tbr1 + neurons f, g, Aldh1 + astrocytes h, i, Olig2 + oligodendrogial lineage cells j, k, and Pdgfra + oligodendrocyte precursors cells l detected 5 days after E13 electroporation, analysis at E18. Data from n = 5 for control and n = 6 mice for Cre for Tbr2 staining; n = 6 for control and n = 5 for cre for Aldh1 staining; and n = 4 per group for Olig2 and Pdgfra staining. Statistical analyses between control and experimental groups performed by ANOVA with Tukey’s post hoc test. Scale bars: 50 μm. Source data are provided as a Source Data file. Data shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns–not significant. Labels: Ctx–cortex, LV–lateral ventricle, VZ–ventricular zone