Fig. 5
Cultured Cic-null cells are oligodendrocyte lineage-biased. a–c Cic-null and control mouse NSCs, cultured in serum-free stem cell proliferation media and analyzed for expression of Nestin, Sox9, Olig2, Gfap, and bIII-Tubulin (Tuj1). Representative images of immunofluorescence staining a; quantitation of cell percentages expressing Nestin, Sox9, and Olig2 b; and western blots for Nestin, Sox9, Olig2, Gfap, and Tuj1, with b-Actin loading control c. d–j Responses of Cic-null and control NSCs to 10-day exposure to lineage-directed differentiation conditions for neurons, astrocytes, and oligodendrocytes. Representative staining and quantitation of Tuj1 + cells d, g in cultures exposed to neuronal-promoting conditions. Representative staining and quantitation of Gfap + cells e, h in cultures exposed to astrocyte-promoting conditions. Representative staining and quantitation of Olig2 + cells as a percentage all cells, Pdgfra + Olig2 + cells as a percentage of Olig2 + cells, and Mbp + Olig2 + cells as a percentage of Olig2 + clls f, i in cultures exposed to oligodendrocyte-promoting conditions, with western blotting j for Olig2, Pdgfra, and Mbp. Data from n = 3 biologic replicates per condition for all experiments. Source data are provided as a Source Data file. Statistical analysis performed by unpaired t test. Scale bar: 10 μm. Data shown as mean ± SD. ns–not significant, **p < 0.01, ****p < 0.0001
