Fig. 2
From: A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy
Rv1468c expresses on mycobacterial surface and mediates Ub binding to Mtb. a Immunoblot analysis of Rv1468c in subcellular fractions of the indicated Mtb strains. b Electron microscopy analysis for localization of Rv1468c immunolabelled with 10 nm gold (black arrows) on ultrathin sections of the mycobacterial strains as indicated. Staining controls for anti-Rv1468c were done with preimmune serum. Asterisks represent the mycobacterial cells. Scale bars, 100 nm. c, d Immunoblot analysis of whole bacterial lysate of the indicated Mtb strains pretreated with (+) or without (–) proteinase K followed by incubation with K632–7 (c) or K482–7 (d) poly-Ub chains (+) at 4 °C for 4 h. Results are representatives from at least three independent experiments
