Fig. 6
From: A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy
Rv1468c is required for host p62 targeting to Mtb in macrophages. a Confocal microscopy analysis for colocalization of Mtb with p62 in BMDMs. BMDMs were infected with the indicated Mtb strains at MOI = 5 for 24 h and were then immunostained using anti-p62 antibody (red). Bacteria (green) were prestained with Alexa Fluor 488 succinimidyl ester before infection. Arrows indicate the colocalization of bacteria with p62. Inserts (enlarged views) show representative mycobacteria colocalized with p62. Scale bars, 20 µm. b Percent colocalization of Mtb with Ub in BMDMs treated as in a for 0–24 h. A total of 100 bacterial cells were counted. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (two-way ANOVA). Results are representatives from three independent experiments (mean ± s.e.m. of n = 4 in b). The source data used in b are provided in Source Data
