Fig. 7
From: A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy
Deletion of Atg5 suppresses Rv1468c-triggered autophagic clearance of Mtb. a Survival of Mtb in macrophages. BMDMs derived from Atg5flox/flox (Atg5fl/fl) or Atg5flox/flox-Lyz-Cre (Atg5LyzM) mice were pretreated with ( + ) or without (–) 50 μM rapamycin (Rapa) for 4 h to induce autophagy, and were then infected with each of the indicated Mtb strains at MOI = 1 for 24 h. P > 0.05, not significant (ns); *P < 0.05; **P < 0.01; ****P < 0.0001 (two-way ANOVA). b, c Confocal microscopy for colocalizations of Mtb with LC3 or LAMP1 in BMDMs. Atg5fl/fl or Atg5LyzM BMDMs were infected with the indicated Mtb strains for 24 h, and were then immunostained using anti-LC3 (b) or anti-LAMP1 (c) antibody (red). Bacteria (green) were prestained with Alexa Fluor 488 succinimidyl ester before infection. Scale bars, 10 µm. d, e Percent colocalizations of the indicated bacterial strains with LC3 (d) or LAMP1 (e) in Atg5fl/fl or Atg5LyzM BMDMs treated as in b and c. A total of 100 bacterial cells were counted. P > 0.05, not significant (ns); ****P < 0.0001 (two-way ANOVA). Results are representatives from at least three independent experiments (mean ± s.e.m. of n = 5 in a; n = 4 in d, e). The source data used in a, d, e are provided in Source Data
