Fig. 2
Spatial map rotation is associated with increased cue exploration. a Examples of control and L7-PP2B mice running tracks during the first 2 min of exploration in consecutives stable sessions, or in sessions associated with spatial map rotation (SSMR). b–g Scatterplot showing the mean distance from the cue (b, c), the % of time in the cue zone (d, e) and the number of entries in the cue zone, normalized by the traveled distance (f, g) in stable sessions and in SSMR. Basal characterization of cue behavior during the 1st session of stable sessions showed a tendency in L7-PP2B mice to explore more the object than controls (mean distance from the cue, U = 1681, p = 0.011; % of time spent in the cue zone, U = 2080, p = 0.44; normalized number of entries in the cue zone, U = 1349, p < 0.001). An increase in cue behavior was observed in L7-PP2B mice, in S2 relative to S1 during SSMR, but not during stable sessions (mean distance from the cue: stable sessions Z = 0.82, p = 0.82; SSMR, Z = 3.24, p = 0.0024; % of time in the cue zone: stable sessions Z = 0.20, p = 0.17; SSMR, Z = 2.37, p = 0.0368; normalized number of entries in the cue zone: stable sessions Z = 0.017, p = 1; SSMR, Z = 2.77, p = 0.011, Wilcoxon Signed-Rank test). h–j S2/S1 ratio of the mean distance from the cue (h), the percentage of time in the cue zone (i), and the normalized number of entries in the cue zone (j) in controls and L7-PP2B mice. In L7-PP2B mice, all parameters were modified in SSMR compared to stable sessions (mean distance from the cue, U = 275, p = 0.010; % of time in the cue zone, U = 281, p = 0.012; normalized number of entries in the cue zone, U = 267, p = 0.0072, Mann–Whitney U test). In control mice, no statistical comparison was applied given the low sample size of unstable cells. p values were adjusted for multiple comparisons with a Bonferroni correction. N and n indicate mice and cell number, respectively. Error bars represent S.E.M.
