Fig. 6

Skin immunisation promotes the early recruitment of ILC1 and monocytes to the FRT. a, b Frequency of ILC1 (CD45+NK1.1+ CD3−) and monocytes (CD45+Ly6C+CD11b+MHCII−) in peripheral blood (a) and FRT tissue (b) at the indicated time points from naive or Ad-CN54 gag skin MA immunised mice (c, d). Representative flow-cytometry profile of CD69 expressed on ILC1 isolated from peripheral blood (c) or FRT tissue (d), 24 h after Ad-CN54 gag immunisation or from naive controls. Data are from three independent experiments (n = 3–10 per group). *p < 0.05, **p < 0.01 by one-way ANOVA, error bars show s.e.m