Fig. 4
From: ALPK1 hotspot mutation as a driver of human spiradenoma and spiradenocarcinoma
Assessment of the MYB-NFIB fusion and p65 expression in adnexal tumors. a Fluorescence in situ hybridization (FISH) imaging of the MYB-NFIB fusion in an adenoid cystic carcinoma and assessment in cylindroma samples. Previous reports have suggested that adnexal tumors, such as cylindomas, carry MYB-NFIB fusions which have been associated with MYB overexpression8. The left panel shows an adenoid cystic carcinoma, which is a positive control for the fusion event. Yellow signal results from the overlap of the green NFIB probe and red MYB probe. Right panel: a representative cylindroma which was fusion negative. b Representative histopathological images of a cylindroma at ×100 magnification, spiradenoma at ×100 magnification, high-grade spiradenocarcinoma at ×200 magnification, and a low-grade spiradenocarcinoma at ×200 magnification. c p65 immunohistochemistry of a CYLD mutant cylindroma (left) and an ALPK1 p.V1092A mutant spiradenoma (right) at ×20 magnification
