Fig. 4
From: Near physiological spectral selectivity of cochlear optogenetics
Quantification of spatial tuning curves (STCs). a Quantification of STCs: STC in response to a 2 kHz pure tone in a non-injected gerbil. Cumulative d′ values are color-coded in a matrix sorted according to electrode number (ordinate) and stimulus intensity (abscissa). The electrode with the lowest threshold (d’ = 1) is defined as the best electrode (BE, white star) and the spread of excitation (SoE) is measured at different activation strengths (exemplary shown for a d′ of 3 at the BE). b BEs as a function of stimulation frequency recorded from non-injected gerbils. Solid line: linear fit of tonotopic slope. Different symbols mark different animals. c BEs as a function of optical stimulation site in virus-injected animals, including mean and standard deviation for each stimulation site. Symbols mark different animals. d BEs as a function of stimulation electrode for monopolar electrical stimulation in non-injected animals, including mean and standard deviation for each electrode. e BEs as a function of stimulating electrode pair for bipolar electrical stimulation in non-injected animals, including mean and standard deviation for each electrode pair. Pearson’s correlation coefficient r and the corresponding p-values were calculated for panel b–e. f–i Spectral spread has been quantified at different d′-values for acoustic (f), optogenetic (g), monopolar (h) and bipolar electric stimulation (i). Box plots indicate minimum (lower) and maximum (upper) whisker, median (center line), as well as 25th percentile and 75th percentile. j Mean and SEM for the spread of excitation upon acoustic, optogenetic, monopolar, and bipolar electric stimulation. Stars indicate statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001), according to repeated-measures ANOVA and post-hoc pairwise comparison tests. Only significant differences have been indicated. Source data of panels b–j is provided as a source data file
