Fig. 8
From: Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells
Intrinsic differences in progenitor cell fate in SC and VSAT. a Principal component analysis performed on the percentage of progenitor cell subsets (determined by flow cytometry) from paired biopsies of subcutaneous (SC, black) and visceral (VS, red) AT according to BMI and waist circumference of n = 23 non-obese and n = 123 obese women. b Spearman correlation between the percentage of −/− cells and MSCA1+ cells or −/CD271+ cells in SCAT (black) and VSAT (red) (n = 23 non-obese and n = 123 obese women). c Myofibroblastic and adipogenic potential of obese SC and VSAT progenitor cells assessed by αSMA/DAPI staining and Bodipy staining, respectively. Representative immunofluorescence analyses performed on three independent donors. Scale bar: 100 µm. d Progenitor cell number per gram of AT in non-obese (n = 23) and obese (n = 123) SC and VSAT, the results are means ± s.e.m. Two-way ANOVA followed by Tukey’s multiple comparison test, **P < 0.01 and ***P < 0.001 comparing non-obese and obese subsets in the same AT location, ###P < 0.001 comparing SC and VS subsets of non-obese or obese individuals. e Distribution of mature adipocytes according to diameter in SC and VS lobules in n = 11 non-obese (upper panel) and n = 71 obese (lower panel) individuals, the results are means ± s.e.m. Two-way ANOVA followed by Sidak’s multiple comparison test, **P < 0.01. f Representative image of dissected lobules of matched SC and VSAT from obese donors and number of lobules per 100 mg of paired SC and VSAT, paired t test, **P < 0.01 (n = 11 independent donors). g Progenitor cell subset repartition in SC (left, black) and VS (right, red) AT from obese patients with (white bars, n = 57) or without (filled bars, n = 66) a metabolic syndrome, the results are means ± s.e.m. One-way ANOVA followed by Sidak’s multiple comparison test, **P < 0.01, ***P < 0.001
