Fig. 4

Voronoï-based colocalization between F-actin regulators and PSD95 in synapses. a Overlay display of the Voronoï diagrams for F-actin regulatory proteins (Abi1, VASP and Abi1 with constitutive Rac1 activation) and PSD95 on 3 different neuronal dendritic spines (left) (scale bar 200 nm). 5 class classification of the localizations (middle). Manders’ coefficients (M^A) and Spearman rank correlation (S^A) computed from the scatterplots of the F-actin regulatory protein localizations (right). Abi1 protein exhibits a much higher colocalization (M^A = 0.85, S^A = 0.71) compared to VASP (M^A = 0.2, S^A = 0.39) or Abi1 with constitutive Rac1 activation (M^A = 0.01, S^A = −0.25). b Manders’ coefficient computed on selected synapses for the different F-actin regulatory proteins (Abi1: M^A = 0.74 ± 0.02 SEM; VASP: M^A = 0.17 ± 0.02 SEM; ArpC5A: M^A = 0.19 ± 0.03 SEM; Abi1 with constitutive Rac1 activation: M^A = 0.15 ± 0.04 SEM). c Spearman rank correlation computed on the same synapses (Abi1: S^A = 0.62 ± 0.02 SEM; VASP: S^A = 0.24 ± 0.03 SEM; ArpC5A: S^A = 0.26 ± 0.03 SEM; Abi1 with constitutive Rac1 activation: S^A = 0.14 ± 0.04 SEM. In all box plots the center line is the median, the square is the mean and the bounds of the boxes are the 75 and 25% percentiles i.e., the interquartile range (IQR)