Fig. 7

Knockdown of lnc-TALC restores TMZ sensitivity in TMZ-resistant GBM xenografts. a Nude mice were orthotopically xenografted with LN229 and 229R GBM cells (5 × 10⁵ cells) and treated intraperitoneally with TMZ (60 mg kg⁻¹ day⁻¹ per mouse) or DMSO (0.3%) every 5 days. b Bioluminescent images of nude mice. c Quantification of bioluminescent imaging signal intensities in nude mice. d Kaplan-Meier survival curve of nude mice is shown. e Nude mice were orthotopically xenografted with 229R GBM cells (5 × 105 cells) transfected with LV-CRISPR-lnc-TALC or LV-scramble and treated intraperitoneally with TMZ (60 mg kg⁻¹ day⁻¹ per mouse) or DMSO (0.3%) every 5 days. f Bioluminescent images of nude mice. g Quantification of bioluminescent imaging signal intensities in nude mice. h Kaplan-Meier survival curve of nude mice is shown. i IHC staining of p-MAPK, p-AKT, p-Stat3 and MGMT in consecutive brain sections of mice orthotopically xenografted with 229R and LN229 GBM cells. Scale bar = 50 μm. j IHC staining of p-MAPK, p-AKT, p-Stat3 and MGMT in consecutive brain sections of mice orthotopically xenografted with 229R GBM cells transfected with LV-CRISPR-lnc-TALC and LV-Scra. Scale bar = 50 μm. Data are presented as the mean ± S.D. P value was determined by one-way ANOVA or Log-rank test. Significant results were presented as NS non-significant, *P < 0.05, **P < 0.01, or ***P < 0.001