Fig. 5
From: A protein complex required for polar growth of rhizobial infection threads
Interaction of VPY and LIN. a–c Live cell confocal images of root hairs from transgenic plants containing pVPY:VPY-GFP and pLjUBQ1:mCherry-LIN showing co-localization of VPY and LIN after inoculation with rhizobia. d, e Live cell images from the root hair tip shown in b, c at two time points (0″ and 3′54″) showing the movement and fusion of VPY-GFP/mCherry-LIN labeled puncta. f–g Yeast two-hybrid assays: between LIN and VPY, MSP domain of VPY and ankyrin repeats of VPY, respectively (f) and between VPY and different domains of LIN (g). The combination of proteins expressed in either the AD vector (pGADT7) or the BD vector (pGBKT7) are indicated alongside the yeast colonies. Diploid yeast cells with a series of 10- fold dilutions were grown on either SD-Leu-Trp (SD-LW) or SD-Ade-His-Leu-Trp (SD-AHLW) media. h Bimolecular Fluorescence Complementation (BiFC) experiments after inoculation with rhizobia. Split Venus fluorescent protein was used, with N-Venus fused to LIN driven by pLjUBQ1 promoter and C-Venus fused to VPY, MSP domain or ankyrin repeats containing domain of VPY driven by VPY promoter. DsRED was used as a transgenic marker. DsRED and GFP images were pseudo coloured in red and green, respectively. n, nucleus; Arrowheads, VPY-GFP/mCherry-LIN puncta. Scale bars, 10 µm
