Fig. 5 | Nature Communications

Fig. 5

From: ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes

Fig. 5The alternative text for this image may have been generated using AI.

ZFVYE21 regulates complement-mediated inflammation in vivo. Coronary artery segments were implanted as interposition grafts in descending aortae of SCID/bg immunodeficient mice who received 200 μL IgG-depleted (n = 4) or intact panel reactive antibody (PRA) sera (n = 4) intravenous prior to graft harvest and immunofluorescence analysis as indicated (a, scale bar: left 200 μm, right 400 μm). Human coronary artery xenografts were pretreated with miltefosine (40 mg/kg once daily intraperitoneally for 3 days) prior to injection of PRA sera. Grafts were harvested 24 h afterwards and analyzed for intimal expression of ZFYVE21 and E-selectin (b, n = 3, scale bar: left 200 μm, right 400 μm). Human coronary artery segments treated with vehicle (n = 8) or miltefosine (n = 8) were exposed to PRA sera and then reparked as interposition xenografts in the descending aortae of SCID/bg immunodeficient mice engrafted with human T cells. Fourteen days posttransplant, neointimal CD4+ T cells were enumerated (c, scale bar: 800 μm), and neointimal and luminal areas were calculated (d, scale bar: 800 μm). **p < 0.001, Student’s t test **p < 0.001

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