Fig. 1
Pericentriolar material 1 (PCM1) binds to GABARAP via a C terminally extended LC3-interacting region (LIR) motif. a Structure of PCM11951–1964 LIR bound to GABARAP (P212121). The PCM1 LIR sequence is shown in orange cartoon with interacting residues shown as sticks. GABARAP is displayed in white cartoon and transparent surface with hydrophobic pocket 1 and 2 colored in pink and blue surfaces, respectively. b, c Close-up view of Q59GAB interaction with main chain carbonyl residues of P1962PCM1 and K1964PCM1 in two different crystal forms (b: P212121; c: P43). d Sequence alignment of human ATG8-family orthologs using ESPript61. Identical (red) and similar residues (yellow) are boxed. Red asterisks and green boxes indicate non-conserved residues between LC3A/B and LC3C/GABARAPs further analyzed in this study. e MYC-TRAP immunoprecipitation (IP) of HEK293A cells expressing MYC-ATG8 constructs and immunoblot with indicated proteins. f Sequence alignment of LIR peptides. The core LIR motif is boxed and aromatic and hydrophobic residues in position Θ0 and Γ3 depicted in blue. Residues in position X6–8 are boxed to highlight proline residues (yellow). Acidic residues are shown in red. g Affinities (Kd values) of LIR peptides to human ATG8 proteins determined by bio-layer interferometry (BLI). Color code indicates fold changes relative to wild-type (WT) GABARAP (data are mean ± s.d., n = 2–4)
