Fig. 3 | Nature Communications

Fig. 3

From: Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins

Fig. 3

The C-terminal region is critical for ULK1 LC3-interacting region (LIR) binding specificity. a, b Mutational peptide array of 24-mer ULK1 peptide covering LIR motif incubated with GST-GABARAP (a) or GST-LC3B (b) and immunoblotted with anti-GST. Each amino acid position was substituted for every other amino acid. c Twenty four-mer peptide array analysis of ULK1 LIR peptides containing point mutations incubated with indicated GST-ATG8 protein and immunoblotted with anti-GST. Each peptide is spotted in triplicates. Mutated residues are highlighted in red. d GST pulldown of HEK293A cells expressing indicated HA-ULK1 constructs and immunoblot with indicated antibodies

Back to article page