Fig. 4
Position X2 in the pericentriolar material 1 (PCM1) core LC3-interacting region (LIR) motif inhibits LC3 binding. a Twenty four-mer peptide array analysis of PCM1-FYCO1 LIR chimera sequences incubated with indicated GST-ATG8 protein and immunoblotted with anti-GST. Each peptide is spotted in triplicates. PCM1 and FYCO1 peptide sequence are highlighted in orange and green, respectively. b Twenty four-mer peptide array analysis of PCM1 LIR containing point mutations incubated with GST-ATG8 protein and immunoblotted with anti-GST. Each peptide is represented in triplicates. Mutated residues are highlighted in red. c Affinities (Kd values) of LIR peptides to ATG8 proteins determined by bio-layer interferometry (BLI). Color code indicates fold changes relative to Kd value of PCM1 WT LIR peptide binding to the corresponding ATG8 protein. (data are mean ± s.d., n = 2). d, e Mutational peptide array of 24-mer PCM1 peptide covering LIR motif incubated with GST-GABARAP (d) or GST-LC3B (e) and immunoblot. Each amino acid position was substituted for every other amino acid
