Fig. 6
Non-conserved GABARAP residues are key to selective LC3-interacting region (LIR) binding. a, c, e Quantification of endogenous pericentriolar material 1 (PCM1) (a), ULK1 (c), and FIP200 (e) binding to indicated GFP-GABARAP constructs expressed and immunoprecipitated by GFP-TRAP from HEK293A cells. Representative immunoblot of GFP-TRAP immunoprecipitation (IP) experiments is shown in Supplementary Fig. 6e. b, d, f Quantification of endogenous PCM1 (b), ULK1 (d), and FIP200 (f) binding to indicated GFP-LC3B constructs expressed and immunoprecipitated by GFP-TRAP from HEK293A cells. Representative immunoblot of GFP-TRAP IP experiments is shown in Supplementary Fig. 6f. Statistical analysis using one-way analysis of variance (ANOVA) test; mean ± s.d.; data from at least three independent experiments. ****p ≤ 0.0001; ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05. g Affinities (Kd values) of LIR peptides to indicated ATG8 proteins measured by bio-layer interferometry (BLI). Color code represents fold changes relative to wild-type (WT) GABARAP and LC3B, respectively (data are mean ± s.d., n = 2–5)
