Fig. 3 | Nature Communications

Fig. 3

From: Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis

Fig. 3

MIRLET7D binds the MiCEE complex and ncRNAs from MIRLET7D targets. a Western blotting (WB) of precipitated proteins after miRNA pulldown (miR-Pd) using nuclear fraction from Ctrl primary human lung fibroblasts (hLF) and biotinylated MIRCTRL or MIRLET7D. Input, 5% of starting material. Beads, control without probe. b Mature MIRLET7D-specific TaqMan assay after chromatin RNA immunoprecipitation (Ch-RIP) using the indicated antibodies and chromatin isolated from Ctrl or IPF hLF. CTNNB (beta-catenin) and IgG were used as negative controls. c qRT-PCR-based analysis of the indicated ncRNAs and cRNAs after miR-Pd from Ctrl or IPF hLF transfected with biotinylated MIRCTRL or MIRLET7D. d In silico analysis of ncRNAs from MIRLET7D target genes (magenta) identified MIRLET7D (green)-binding sites. MFE, minimum free energy. In all bar plots, data are shown as mean (SD) (n = 3 biologically independent experiments). Asterisks in all plots, P-values after unpaired t-test, two tailed, ***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant. Source data are provided as a Source Data file

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