Fig. 4
Loss-of-function of MiCEE components increased fibrosis hallmarks. a–d LOF of MIRLET7D by an antagomir (antiMIRLET7D), EXOSC10 by a short hairpin RNA construct (shEXO10), and EZH2 by the small-molecule inhibitor UNC-1999 (EZH2inh) in Ctrl human primary lung fibroblasts (hLF) increased cell migration (a, b), cell proliferation (c), and protein levels of fibrotic markers (d) to similar levels as in IPF hLF. Scratch migration assays (a) of Ctrl or IPF hLF quantified as the migratory distance 12 h after scratch (see Supplementary Fig. 3a). Quantification of Transwell invasion assays followed by hematoxylin and eosin (H&E) stain (b) of Ctrl or IPF hLF (see Supplementary Fig. 3b). BrdU incorporation assays (c) using Ctrl or IPF hLF. WB of whole-cell protein extracts from Ctrl or IPF hLF using the indicated antibodies (d). In all bar plots, data are shown as mean (SD) (n = 3 biologically independent experiments for a and c; n = 6 biologically independent experiments for b). Asterisks in all plots, P-values after unpaired t-test, two tailed, ***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant. Source data are provided as a Source Data file
