Fig. 7
The transcriptional function of CBFB is critical for breast tumor suppression. a A Venn diagram showing genes (identified by RNAseq) co-regulated by CBFB and RUNX1. b Kaplan-Meier curve showing 10-gene signature upregulated by CBFB and RUNX1 predicts disease-free survival of breast cancer patients. c IB showing the upregulation of Notch intracellular domain (NICD) of NOTCH3 in CBFB_KO and RUNX1_KO cells. d ChIPseq of RUNX1 showing the binding of RUNX1 on the NOTCH3 locus. e Anchorage independent growth assays of double knockout (DKO) of CBFB (C) and NOTCH3 (N) or DKO of RUNX1(R) and NOTCH3(N). n = 3 (biological); two asterisks, p value < 0.01 (single KO vs. double KO comparisons, two-tailed t test). f Representative IHC images showing the protein levels of CBFB, RUNX1, and NOTCH3 in human breast tumors and normal tissues. g IB showing the effect of exogenous expression of CBFB on RUNX1 and NOTCH3 expression in MCF7 and BT474 cells. h Weight and (i) volume of orthotopically transplanted tumors from MCF7 cells that were transduced with an empty vector (EV) or a lentiviral vector expressing CBFB (CBFB). Tumor incidence is shown on the top
