Fig. 2
From: Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system
RIBEYE-tagRFP-T mice enable visualization of ribbons in live retina. a A schematic drawing of the RIBEYE-RIBEYE:tagRFP-T transgene. b Volumetric view of a two-photon reconstruction of RIBEYE-tagRFP-T live retina. OPL outer plexiform layer. IPL inner plexiform layer. c Vertical section of CtBP2 labeling in control (ctrl) retina. d Vertical section of CtBP2 labeling, RIBEYE-tagRFP-T (tagRFP-T), and overlay of both channels. e Colocalization of CtBP2 labeling and RIBEYE-tagRFP-T signal on a type 6 BC axon terminal. f Comparison of number of CtBP2 puncta on T6 BC axon terminal in control (ctrl; Grm6-YFP; eifht cells from eight retinas) and RIBEYE-tagRFP-T (RE-RFP) (six cells from four retinas) mice. g An example of light-evoked EPSC on ON-alpha RGCs in control and RIBEYE-tagRFP-T mice. Quantification of the amplitude of the transient (h) and sustained (i) components of the light-evoked EPSC. (f, h, and i: n number of cells, from three retinas for each group). P21 mice were used in all experiments. Data are presented as mean ± sem
