Fig. 5 | Nature Communications

Fig. 5

From: Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system

Fig. 5

PSD95 puncta are more stable when apposed to ribbons. a RGCs were biolistically labeled with CFP together with PSD95-YFP. Live-cell imaging: every 2 h, up to 10 h. b From top to bottom: An example of PSD95 formation with (w/) or without (w/o) RIBEYE-tagRFP-T and PSD95 elimination with or without RIBEYE-tagRFP-T puncta. c The rate of PSD95 puncta formation with and without RIBEYE-tagRFP-T puncta at P10 and P40. d Survival rate of PSD95 puncta with and without RIBEYE-tagRFP-T puncta at P10 and at P40. Five live imaging experiments are performed at both P10 (five cells from four retinas) and P40 (four cells from two retinas). Data are presented as mean ± sem

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