Fig. 1

Full induction of p21 is Nup155-dependent. a Protein fold-changes (blue and grey dots) including p53 targets (red dots) upon Nup155 (orange dot) knockdown compared to the control siRNA (AS) condition are shown as log2 ratios. p21 is highlighted in green as a key effector of the p53 response. Overall 3523 proteins could be quantified by quantitative mass spectrometry in HepG2 cells treated with Nutlin-3a for 24 h. b HepG2 cells were treated either with control siRNA (AS) or three different Nup155 siRNAs (Nup155#1, Nup155#2, and Nup155#3) for 72 h and p53 was induced by adding Nutlin-3a for 24 h. Cell extracts were analysed by immunoblotting with indicated antibodies (upper panel). Densitometric quantification of immunoblots derived from three independent experiments is shown in the lower panel and normalised to the control siRNA (AS) condition. c HepG2 cells were treated either with control siRNA (AS) or two different Nup35, Nup93, and Nup188 siRNAs (Nup35#1, Nup35#2, Nup93#1, Nup93#2, Nup188#1, and Nup188#2) for 72 h. Cells were harvested upon 24 h of Nutlin-3a treatment and extracts were analysed by immunoblotting with the indicated antibodies. For corresponding densitometric analyses see Supplementary Figure 1B-D. d Protein fold-changes of NPC components (purple dots) upon Nup155 knockdown (orange dot) corresponding to the conditions described in a. e Representative confocal microscopy pictures of MAb414 immunofluorescence staining of HepG2 cells treated as indicated. DAPI was used for DNA labeling. Scale bar = 10 µm. *p < 0.05, **p < 0.01, ***p < 0.001 (Student′s t-test). Data are presented as mean ± stdv. Source data are provided as a Source Data file