Fig. 9
Partial rescue of PNP closure in Axd/Axd embryos by myosin II inhibition. a Diagram of experimental approach and b representative images of embryos, contained within the yolk sac after DiI marking and after 8 h culture (scale bars represent 500 µm). c, d Light (upper row) and confocal (lower row) images of the PNP region (caudal end oriented upwards) after culture in the presence of vehicle or 50 µM blebbistatin (effective dose is higher than in Fig. 8 as the yolk sac is open). Asterisks indicate the position of the DiI mark and arrows indicate the neural tube closure point (scale bars represent 100 µm). e Progression of closure is diminished in Axd/Axd embryos compared to other genotypes when cultured in vehicle only, but not after culture with blebbistatin. f PNP length is greater in Axd/Axd embryos than in other genotypes in both treatment groups but blebbistatin-treated mutants have a smaller PNP than vehicle-treated mutants (n > 6 per group; **p < 0.01, ***p < 0.0001, One-Way ANOVA). Source data are provided as a Source Data file
